Spermidine 3HCl

Description

Spermidine 3HCl for Sale

All Iron Mountain Labz products are only intended for laboratory research use and are not approved for human consumption.

Overview of Spermidine 3HCl

Spermidine trihydrochloride (CAS 334-50-9; spermidine 3HCl) is a polyamine salt — the water-soluble trisalt form of the endogenous natural polyamine spermidine. A polyamine is a chain of nitrogen and carbon atoms with multiple amine groups; polyamines are not amino acids and contain no amino acid bonds or peptide linkages. Spermidine (free base) has molecular formula C7H19N3; MW: 145.20 g/mol (free base), 254.63 g/mol as the trihydrochloride salt (C7H19N3·3HCl). IUPAC name: N-(3-aminopropyl)-1,4-butanediamine. Spermidine 3HCl is not a SARM — it has no androgen receptor interaction in established preclinical models. It is not a peptide — it is a polyamine: a simple nitrogen-carbon chain molecule, containing no amino acid residues and no peptide bonds. It is naturally found in all living cells. 

Spermidine is biosynthesized intracellularly from putrescine and decarboxylated S-adenosylmethionine (dcSAM) via spermidine synthase (SRM; EC 2.5.1.16) and functions as the exclusive aminopropyl donor for eIF5A hypusination — a conserved post-translational modification essential for eukaryotic translation elongation and a key node in autophagy regulation. Its research significance has expanded significantly following landmark findings linking spermidine supplementation to autophagy enhancement and longevity across multiple model organisms.

This product is not approved by the FDA for any therapeutic indication beyond its DSHEA dietary supplement context, including injection or any clinical administration. In this research-grade formulation, it is intended for laboratory and research purposes only. It is not a dietary supplement or consumer product in this form. Restricted to qualified researchers and licensed laboratory institutions. IRB guidance is required for clinical research; IACUC compliance is required for preclinical animal research. Highly hygroscopic — reseal immediately after use; store with desiccant.

Chemical Properties

Section	Details
CAS Number	334-50-9 (trihydrochloride salt)
Chemical Class	Polyamine Salt
Classification — NOT a SARM	No androgen receptor interaction in established preclinical models
Classification — NOT a Peptide	A polyamine: chain of nitrogen and carbon atoms with no amino acid bonds; naturally found in all living cells
Regulatory Status	Research-grade material – for laboratory use only. Not sold as a dietary supplement by Iron Mountain Labz. Note: Spermidine is available as a consumer dietary supplement from other vendors; this research-grade formulation is analytically distinct and not intended for consumption.
Market Price Reference	$765/100g research grade; $30–$50/supplement grade
Molar Mass	254.63 g/mol (trihydrochloride salt); 145.20 g/mol (free base)
Chemical Formula	C7H19N3·3HCl
IUPAC Name	N-(3-aminopropyl)butane-1,4-diamine trihydrochloride
Synonyms	Spermidine trihydrochloride; spermidine 3HCl; N-(3-aminopropyl)-1,4-diaminobutane 3HCl; SPD·3HCl
PubChem CID	1102 (spermidine free base; 3HCl salt registered separately by suppliers)
Physical Form	White crystalline hygroscopic powder
Purity	≥99% (HPLC-UV; potentiometric titration)
Solubility	Freely soluble in water (>200 mg/mL); aqueous solutions mildly acidic (pH ~5.5–6.5 at 10% w/v)
Storage	2–8°C; tightly sealed with desiccant; protect from moisture — highly hygroscopic; reseal immediately after each use
Shelf Life	24 months from manufacture date under recommended conditions
Classification	Research Use Only (RUO)

Spermidine 3HCl’s Mechanism of Action in Research Models

Within polyamine biology and autophagy research, spermidine’s most well-characterized mechanistic role is as the exclusive aminopropyl donor for eIF5A hypusination. This two-step post-translational modification — conserved from archaea to mammals — proceeds as follows: Step 1, deoxyhypusine synthase (DHS; DHYS; EC 2.5.1.46) cleaves the aminobutyl moiety of spermidine and covalently transfers it to the ε-amino group of a specific lysine residue (Lys50 in human eIF5A-1) on the eukaryotic translation initiation factor 5A (eIF5A) protein, forming the deoxyhypusine intermediate and releasing 1,3-diaminopropane; Step 2, deoxyhypusine hydroxylase (DOHH; EC 1.14.99.29) hydroxylates the deoxyhypusine at the C2 position, completing the hypusinated eIF5A modification. Hypusinated eIF5A is required for ribosomal transit through polyproline stretches and rare-codon clusters on mRNAs encoding autophagy regulators including ATG3, ATG12, and TFEB.

Hofer et al. (2024) demonstrated in Nature Cell Biology that spermidine is essential for fasting-mediated autophagy and longevity across multiple model organisms — yeast, C. elegans, Drosophila, mice, and human volunteers — via the polyamine-hypusination axis, and that pharmacological blockade of endogenous spermidine synthesis abrogated fasting-induced autophagy (DOI). In complementary mechanistic work, Wolff et al. (2007) characterized the evolutionary progression and strict enzymatic specificity of the hypusine modification pathway in Amino Acids, demonstrating that DHS exhibits narrow specificity toward spermidine as aminopropyl donor and that eIF5A hypusination is essential for eukaryotic cell proliferation (DOI). Data remains limited, and findings are not consistent across all experimental model systems.

Risk & Handling

Risk Tier: LOW-MODERATE

Spermidine 3HCl is an endogenous naturally occurring polyamine salt. No established acute human toxicity data at laboratory handling concentrations. Primary hazards are hygroscopicity and mild irritant potential:

• Hygroscopic hazard: Absorbs atmospheric moisture rapidly — reseal container immediately after each use; include desiccant in storage; weigh quickly in low-humidity conditions
• Irritant potential: The triamine HCl salt can be mildly irritating to skin, eyes, and respiratory mucosa at high concentrations — avoid inhalation of powder and direct eye contact
• Aqueous solutions: Prepare fresh solutions where possible; store at −20°C for no longer than 1 month; avoid bacterial contamination (no preservatives)
• pH: Concentrated aqueous solutions are mildly acidic (~pH 5.5–6.5 at 10% w/v); adjust to physiological pH as appropriate for biological assay systems
• PPE: Nitrile gloves, lab coat, and safety eyewear for all handling operations
• No human safety data established for this research-grade formulation. Contact help@ironmountainlabz.com for the Safety Data Sheet.

Why Buy Spermidine 3HCl from Iron Mountain Labz?

• Purity: ≥99% by HPLC-UV and potentiometric titration per lot
• Identity: confirmed by ¹H-NMR and ESI-MS; chloride content verified consistent with 3HCl stoichiometry per lot
• Microbial: total viable count ≤100 CFU/g
• Heavy metals: Pb ≤0.5 ppm; As ≤0.5 ppm (ICP-MS)
• Certificate of Analysis available on request per lot
• For queries, complaints, or support, please contact help@ironmountainlabz.com 

FAQs

Q1: What is spermidine chemically, and why is it not a SARM or peptide?
Spermidine is a naturally occurring polyamine — a simple triamine molecule (H2N-(CH2)4-NH-(CH2)3-NH2) built from a chain of nitrogen and carbon atoms. It has no amino acid residues and no peptide bonds, making it definitively not a peptide. It has no androgen receptor interaction in established preclinical models, making it definitively not a SARM. It is the endogenous aminopropyl donor for eIF5A hypusination and is found in all living cells.

Q2: What distinguishes research-grade Spermidine 3HCl from supplement-grade spermidine?
Consumer supplement spermidine (typically $30–$50/month, often from wheat germ extract) is sold for general wellness purposes without the analytical characterization required for mechanistic laboratory research. Iron Mountain Labz supplies research-grade spermidine 3HCl at ≥99% purity with per-lot identity confirmation by NMR, MS, and potentiometric titration — meeting the analytical standards required for eIF5A hypusination assays, polyamine pathway research, and autophagy biology experiments. This product is not a dietary supplement in this research-grade formulation.

Q3: What research applications has spermidine been investigated in?
In preclinical and in vitro laboratory settings: eIF5A hypusination assay systems (DHS enzyme activity, deoxyhypusine formation), autophagy flux measurement (LC3-II/I ratio, p62 degradation), polyamine pathway research (putrescine/spermidine/spermine biosynthesis), fasting-mediated longevity models in C. elegans and Drosophila, and cardiovascular aging research in preclinical rodent models. All applications strictly in laboratory and preclinical contexts.

Q4: What storage precautions are critical for Spermidine 3HCl?
Spermidine 3HCl is highly hygroscopic — store tightly sealed with desiccant at 2–8°C. Prepare aqueous solutions fresh and store at −20°C for no longer than one month to prevent degradation. Avoid moisture exposure during weighing by working quickly and immediately resealing the container. No human safety data has been established for this research-grade formulation.

Q5: How is Spermidine 3HCl used in eIF5A hypusination research assays?
Spermidine 3HCl serves as the aminopropyl donor substrate in cell-free deoxyhypusine synthase (DHS) enzyme assays and in cell culture supplementation and depletion experiments. DFMO (difluoromethylornithine, an ornithine decarboxylase inhibitor) is used as a polyamine depletion control to establish spermidine dependency. Downstream readouts of eIF5A hypusination status are assessed by western blot (anti-hypusine antibody) and downstream autophagy flux markers (LC3-II, p62).

References

1. Hofer SJ, Daskalaki I, Bergmann M, et al. Spermidine is essential for fasting-mediated autophagy and longevity. Nat Cell Biol. 2024;26(9):1571–1584. PMID: 39117797.
   https://pubmed.ncbi.nlm.nih.gov/39117797/ 
2. Wolff EC, Kang KR, Kim YS, Park MH. Posttranslational synthesis of hypusine: evolutionary progression and specificity of the hypusine modification. Amino Acids. 2007;33(2):341–350. PMID: 17476569.
   https://pubmed.ncbi.nlm.nih.gov/17476569/ 
3. Madeo F, Eisenberg T, Pietrocola F, Kroemer G. Spermidine in health and disease. Science. 2018;359(6374):eaan2788. PMID: 29371440 https://pubmed.ncbi.nlm.nih.gov/29371440/